rabbit anti human adiponectin polyclonal antibody  (Proteintech)

Journal: Journal of Atherosclerosis and Thrombosis

Article Title: A Novel ELISA System for Measuring Modified LDL-Adiponectin Complex

doi: 10.5551/jat.65377

Figure Lengend Snippet: (A) Cumulative concentration response curve of methylcholine-induced vasodilation in pre-constricted (with U46619) mesenteric arteries incubated overnight without stimulus (white), 50 µg/mL of oxLDL (red), 30 µg/mL of AN (green), or 30 µg/mL of AN plus 50 µg/mL of oxLDL (blue). (B) Summary graph of the maximum response (Emax) obtained from concentrations curves shown in (A). Vessels were obtained from rats ( n = 6-13). (C) Cumulative concentration response curve of methylcholine-induced vasodilation in pre-constricted (with U46619) mesenteric arteries incubated overnight without stimulus (white) or with 50 µg/mL of oxLDL (red), 30 µg/mL of AN plus 50 µg/mL of oxLDL (blue), 30 µg/mL of AN plus 10 µg/mL of TS20 (purple), or 10 µg/mL of the IgG isotype (black). (D) Summary graph of the maximum response (Emax) obtained from concentrations curves shown in C. Vessels from n = 6-7 rats. Values are shown as the mean±SEM. ＊ p ＜0.05; ＊＊ p ＜0.01; ＊＊＊ p ＜0.001. ns, non-significant.

Article Snippet: The wells were washed three more times with PBS, and the plates were incubated for 1 h at 25°C with 1.0 μg/mL anti-adiponectin antibody (BioVendor, RD181023100) in 1% (w/v) casein-Na blocking solution.

Techniques: Concentration Assay, Incubation

Journal: Journal of Atherosclerosis and Thrombosis

Article Title: A Novel ELISA System for Measuring Modified LDL-Adiponectin Complex

doi: 10.5551/jat.65377

Figure Lengend Snippet: (A) Schematic representation of recombinant human T-cadherin proteins fused with Myc-His-tag. (B) Coomassie brilliant blue (CBB) staining and western blot analysis of purified T-cadherin proteins. (C) Binding of recombinant AN to immobilized T-cadherin in ELISA was determined by anti-AN antibody. (D) Binding of recombinant AN−oxLDL complex to immobilized LOX-1 in ELISA was determined by anti-AN antibody. s.p., signal peptide; EC, extracellular cadherin.

Article Snippet: The wells were washed three more times with PBS, and the plates were incubated for 1 h at 25°C with 1.0 μg/mL anti-adiponectin antibody (BioVendor, RD181023100) in 1% (w/v) casein-Na blocking solution.

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant, Staining, Western Blot, Purification, Binding Assay

Journal: Journal of Atherosclerosis and Thrombosis

Article Title: A Novel ELISA System for Measuring Modified LDL-Adiponectin Complex

doi: 10.5551/jat.65377

Figure Lengend Snippet: Correlation between total-AN and high-molecular weight (HMW)-AN (left), total-AN and T-cadherin bindable adiponectin (Tcad-AN) (middle), HMW-AN and Tcad-AN (right).

Article Snippet: The wells were washed three more times with PBS, and the plates were incubated for 1 h at 25°C with 1.0 μg/mL anti-adiponectin antibody (BioVendor, RD181023100) in 1% (w/v) casein-Na blocking solution.

Techniques: Enzyme-linked Immunosorbent Assay, High Molecular Weight

Journal: Journal of Atherosclerosis and Thrombosis

Article Title: A Novel ELISA System for Measuring Modified LDL-Adiponectin Complex

doi: 10.5551/jat.65377

Figure Lengend Snippet: (A) Serum levels of AN, T-cadherin bindable adiponectin (Tcad-AN), oxidized LDL (oxLDL) and LOX-1-ligand containing apoB (LAB) in patients on hemodialysis (HD; n = 35) and control subjects ( n = 35). The error bars represent the mean±SD of the values obtained. (B) Comparison of the proportion of MAC-high between HD and controls. MAC-high and -low groups were divided by the median of the MAC values of 70 subjects.

Article Snippet: The wells were washed three more times with PBS, and the plates were incubated for 1 h at 25°C with 1.0 μg/mL anti-adiponectin antibody (BioVendor, RD181023100) in 1% (w/v) casein-Na blocking solution.

Techniques: Comparison, Control

Journal: Journal of Atherosclerosis and Thrombosis

Article Title: A Novel ELISA System for Measuring Modified LDL-Adiponectin Complex

doi: 10.5551/jat.65377

Figure Lengend Snippet: The error bars represent mean±SD.

Article Snippet: The wells were washed three more times with PBS, and the plates were incubated for 1 h at 25°C with 1.0 μg/mL anti-adiponectin antibody (BioVendor, RD181023100) in 1% (w/v) casein-Na blocking solution.

Techniques: Comparison

Journal: Bone & Joint Research

Article Title: Adiponectin inhibits fibrosis of the palmar aponeurosis in Dupuytren’s contracture in male patients

doi: 10.1302/2046-3758.128.BJR-2022-0449.R1

Figure Lengend Snippet: Primer sequences for quantitative real-time polymerase chain reaction.

Article Snippet: The sections were then incubated overnight at 4°C with a rabbit anti-human adiponectin polyclonal antibody (21613-1-AP, Proteintech, USA; dilution 1:200) and a rabbit anti-human leptin polyclonal antibody (ab16227, Abcam, USA; dilution 1:100).

Techniques:

Journal: Bone & Joint Research

Article Title: Adiponectin inhibits fibrosis of the palmar aponeurosis in Dupuytren’s contracture in male patients

doi: 10.1302/2046-3758.128.BJR-2022-0449.R1

Figure Lengend Snippet: a) Adiponectin gene expression was significantly decreased in the Dupuytren’s contracture group compared to that in the carpal tunnel syndrome (CTS) group (p < 0.001) (n = 8). Gene expression was normalized against the CTS. All results are expressed as means and standard error. b) Fluorescence immunohistochemical staining showing decreased protein expression in the Dupuytren’s contracture group compared to the CTS group (20×). H&E, haematoxylin and eosin.

Article Snippet: The sections were then incubated overnight at 4°C with a rabbit anti-human adiponectin polyclonal antibody (21613-1-AP, Proteintech, USA; dilution 1:200) and a rabbit anti-human leptin polyclonal antibody (ab16227, Abcam, USA; dilution 1:100).

Techniques: Gene Expression, Fluorescence, Immunohistochemical staining, Staining, Expressing

Journal: Obesity (Silver Spring, Md.)

Article Title: Adiponectin isoform patterns in ethnic-specific ADIPOQ mutation carriers: The IRAS Family Study

doi: 10.1002/oby.21892

Figure Lengend Snippet: Phenotypic summaries of IRASFS Hispanic and African American participants

Article Snippet: Primary rabbit polyclonal antibodies against mouse and human adiponectin were obtained from Biovendor R&D Products (Asheville, NC).

Techniques:

Journal: Obesity (Silver Spring, Md.)

Article Title: Adiponectin isoform patterns in ethnic-specific ADIPOQ mutation carriers: The IRAS Family Study

doi: 10.1002/oby.21892

Figure Lengend Snippet: (A) IRASFS Hispanic American and (B) IRASFS African American serum samples blotted under native conditions. Carriers of the G45R mutation (A; Lanes 1–5) and the R55C mutation (B; Lanes 8, 10, and 11) show a distinctively different isoform pattern from Hispanic (A; Lanes 6–13) and African American (B; Lanes 1–7, 9, 12, 13) non-carriers. The HMW isoform is drastically reduced or undetectable in all mutation carriers, and the MMW isoform is also markedly decreased. However, there is a corresponding increase in the LMW isoform. It is worth noting that samples in panel A, lanes 6–8 are from Hispanic American non-carriers, low (RIA) adiponectin levels. These samples display the same isoform pattern as the Hispanic American normal (RIA) adiponectin level non-carriers (A; Lanes 9–13).

Article Snippet: Primary rabbit polyclonal antibodies against mouse and human adiponectin were obtained from Biovendor R&D Products (Asheville, NC).

Techniques: Mutagenesis

Journal: Obesity (Silver Spring, Md.)

Article Title: Adiponectin isoform patterns in ethnic-specific ADIPOQ mutation carriers: The IRAS Family Study

doi: 10.1002/oby.21892

Figure Lengend Snippet: The mean ratio of the high molecular weight isoform to total adiponectin levels. Carriers of the G45R and R55C variants have markedly smaller HMW to total adiponectin ratios than non-carriers. Interestingly, the difference between carriers and non-carriers is more pronounced in the Hispanic Americans than in the African Americans included in this study.

Article Snippet: Primary rabbit polyclonal antibodies against mouse and human adiponectin were obtained from Biovendor R&D Products (Asheville, NC).

Techniques: Molecular Weight